한국인 성인남성 사체에서 시행한 인중 구륜근 섬유들의 효소-조직화학적 분석
Enzyme-Histochemical Study of Philtral Orbicularis Oris Muscle Fiber Types in Korean Male Cadaver
유명숙, 박정민, 이희수, 이석근, 강지영, 어미영, 이종호, 김성민,
소속 상세정보
유명숙 ( Yu Myung-Sook ) - 서울대학교 치의학대학원 구강악안면외과학교실
박정민 ( Park Jung-Min ) - 강릉원주대학교 치과대학 구강악안면외과학교실
이희수 ( Lee Hee-Su ) - 강릉원주대학교 치과대학 구강해부학교실
이석근 ( Lee Suk-Keun ) - 강릉원주대학교 치과대학 구강병리학교실
강지영 ( Kang Ji-Young ) - 서울대학교 치의학대학원 구강악안면외과학교실
어미영 ( Eo Mi-Young ) - 서울대학교 치의학대학원 구강악안면외과학교실
이종호 ( Lee Jong-Ho ) - 서울대학교 치의학대학원 구강악안면외과학교실
김성민 ( Kim Soung-Min ) - 서울대학교 치과대학 구강악안면외과학교실
KMID : 0952820090120020047
Abstract
The orbicularis oris muscle (OOM) is a very important muscle that originate from the second branchial arch and is innervated by the facial nerve. The aim of this study was to elucidate distribution types of two muscle fibers that composing OOM by using enzyme-histochemical examinations and tried to make a basis for a clinical application. The fresh frozen tissues from the superior and inferior portions of the OOM were taken from post mortem 65-year-old Korean male adult. Total five different sagittal sections were used on the midline of the philtrum, the middle portion of lower lip, the mouth corner, and each midlateral side of upper and lower mouth. We used enzyme-histochemical staining such as Periodic Acid-Schiff (PAS), Succinic Dehydrogenase (SDHase), reduced Nicotinamide Adenine Dinucleotide-Tetrazolium Reductase (NADH-TR), Adenosine Triphosphatase (ATPase) in pH 9.4, 4.6 and 4.3, and Modified Gomori Trichrome. There were about 30.24 % type 1 muscle fiber and 65.40 % type 2 muscle fiber in the midline of the philtrum (p < 0.05). Enzyme-histochemical staining is very useful and innovative method to elucidate characteristics of muscle fibers. We expect that chiloplasty and reconstruction of the lip portions for cleft lip patients, based on these results, are better to recovery function and aesthetic. However, we have some problems as an intramuscular variability and the inter-individual variation etc. Therefore we have to make progress these studies continuously to overcome these problems.
키워드
Orbicularis oris muscle;Enzyme histochemistry;Fresh cadaver;Muscle fiber type;Lip reconstruction
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